Thx for the Infos guys!
I finally made my Backup. Here is how:
On a different PC (WIN8)
install Virtualbox.
download the Freedos 1.1 Installer CD (
http://www.freedos.org/download/download/fd11src.iso)
get the Disk Number (Computer Managment -> Disk Managment)
make a link for a bootable HDD for the Target PC (
http://www.yourhelpcenter.de/2010/02/ei ... stallieren)
in an elevated (as Admin) shell go to the VB folder (cd "C:\Program Files\Oracle\VirtualBox")
create the link in the shell; X = Disk Number (VBoxManage internalcommands createrawvmdk -filename c:\realhdd.vmdk -rawdisk \\.\PhysicalDriveX)
Make a new VM with the raw HDD and install Freedos on it (use the FreeDos iso)
Swap the Disk into the target PC, make a backup, swap it again and then copy it somewhere safe

I hope the procedure is understandable