I haven't seen that specific call to int 13h before. Depending on what you are trying to reverse-engineer, the code could be in the BIOS (real or virtualised), or in a software int 13h handler extension, that is loaded after boot, or .... (You know what you are doing - you haven't told us that information, so I won't try to guess.

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If I was trying to find out what that code did, I would trace the call flow for the int 13h handler. There is usually the equivalent of some type of "switch-like" statement early in the handler for different ah values - then investigate what happens with ah=0x54